The changing epidemiology of VanB Enterococcus faecium in Poland

Transferable, plasmid-mediated vanB-type glycopeptide resistance in Enterococcus faecium.

An approximately 60-kb transferable, vanB-carrying plasmid has been identified in a clinical Enterococcus faecium strain. A similar plasmid has been observed in an unrelated E. faecium strain, suggesting that plasmid transfer of vanB operons occurs in nature and plays a role in the dissemination of VanB-type resistance among strains of E. faecium.

VanB phenotype-vanA genotype Enterococcus faecium with heterogeneous expression

Nonconjugative transposition of the vanB-containing Tn5382-like element in Enterococcus faecium.

The vanB2 operon encoding glycopeptide resistance is an integral part of the putative conjugative transposon Tn5382. Characterization of clinical glycopeptide resistant derivatives from an epidemic ampicillin-resistant Enterococcus faecium strain showed precise chromosomal or plasmid insertions of a vanB2-containing Tn5382-like element. Conjugative transposition of the Tn5382-like element was n...

Emergence of VanB phenotype-vanA genotype Enterococcus faecium clinical isolate in Bulgaria.

anA glycopeptide resistance is characterized by acquired nducible resistance to both vancomycin and teicoplanin, hereas the VanB phenotype is characterized by variable evels of resistance to vancomycin but susceptibility to eicoplanin.1 Here we report a vancomycin-resistant Enterooccus faecium (VREFm) strain with VanB phenotype-vanA enotype incongruence. In the course of antimicrobial susceptib...

Peptidoglycan structure of Enterococcus faecium expressing vancomycin resistance of the VanB type.

Resistance to glycopeptide antibiotics in enterococci is due to the synthesis of UDP-MurNAc-tetrapeptide-D-lactate (where Mur is muramic acid) replacing the normal UDP-MurNAc-pentapeptide precursor. The peptidoglycan structures of an inducible VanB-type glycopeptide-resistant Enterococcus faecium, D366, and its constitutively resistant derivative, MT9, were determined. Using HPLC, 17 muropeptid...